Database : HANSEN Search on : MYCOBACTERIUM LEPRAE/ULTRAEST [Subject descriptor] References found : 21 [refine] Displaying: 1 .. 20   in format [Detailed]

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Id: 19783 Author: Silva, Manuel T; Portaels, Françoise; Macedo, Paula M. Title: New data onthe ultrastructure of the membrane of Mycobacterium leprae. Source: Int J Lep;57(1):54-64, Mar. 1989. ^bilus, ^btab, ^bgraf. Abstract: In previous reports on the ultrastructure of Mycobacterium leprae, we described the occurrence of symmetric membranes in normal-looking bacilli from fresh or frozen samples primarily fixed with aldehydes. In those reports we admitted that such a symmetric profile, which is not found in the other normal mycobacteria, would not represent the structure of the normal membrane of the leprosy bacillus. We, therefore, re-analyzed the ultrastructure of the membrane of M. leprae. In the present work the micromorphology of the M. leprae membrane was studied by transmission electronmicroscopy after the fixation of fresh samples by OsO4 plus calcium followed by glutaraldehyde plus formaldehyde and calcium followed by uranyl acetate. The study of samples from two patients with lepromatous (LL) leprosy, three armadillos with natural leprosy, and one nude mouse with experimental leprosy showed that normal-looking bacilli present in lead-stained sections had asymmetric membranes with a thickness of 6.49 +/- 0.36 nm. These membranes showed periodic acid-Schiff (PAS)-positive components exclusively located in the outer half of the bilayer. We demonstrated that the symmetric profile of the M. leprae membrane described in our previous reports corresponds, as admitted in those reports, to an abnormal membrane structure. Such an abnormality was now found to result from the use of primary fixation with aldehydes or of samples stored frozen before fixation. These results indicate that, although ultrastructurally similar to that of the other mycobacteria, the membrane of M. leprae has a peculiar sensitivity to fixation by aldehydes. Such a characteristic, which was not found in M. lepraemurium, M. aurum, M. avium, and M. tuberculosis H37Ra, must reflect a unique membrane molecular structure, which is presently unknown^ien. Descriptors: Mycobacterium leprae/ultraest Mycobacterium avium/ultraest Membrana Celular/ultraest Limits: Animais Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1989/pdf/v57n1/v57n1a08.pdf / en Location: Br191.1

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Id: 19419 Author: Salamatina, Olga S; Dyachina, Margarita N; Juscenko, Anatoly A; Parshin, Michail P; Chernoyusova, Larisa N. Title: Comparative characteristics of antigenic profile of M. leprae and M. lufu. Source: Int. J. Lepr;69(1):39-43, Mar., 2001. ilus, tab. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/ultraest Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n1/v69n1cor04.pdf / en Location: BR191.1

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Id: 19248 Author: Shetty, Vanaja Prabhakar; Antia, Noshir Hormusji. Title: Light and ultrastructural study of sciatic nerve lesions induced using intraneural injection of viable mycobacterium leprae in normal and immunosuppressed swiss white mice. Source: Int. J. Lepr;70(1):25-33, Mar.,2002. ilus, tab. Abstract: Freshly harvested M. leprae were microinjected into the sciatic nerves of nonimmunosuppressed (non-TR) and immunosuppressed (TR) mice using the technique described by Wisniewski and Bloom. The lesions thus induced, on bypassing the blood-nerve barrier, were biopsied at regular intervals beginning 24 hr and followed up to one year. The fate of M. leprae and the ensuing inflammation and nerve damage were studied using light and electron microscopy. The lesions in both non-TR and TR mice at 24 hr showed an influx of polymorphonuclear leukocytes and an increase in mast cells. The influx and peaking of lymphocytes were delayed by two weeks and 6 weeks, respectively, in TR mice, but the density of lymphocytes at the peak intervals was comparable in both. The plasma cells denoting the humoral response were seen in both, but there was a delay of 3 weeks in non-TR mice. The lesions in non-TR mice showed differentiation of macrophages into epithelioid cells and the formation of giant cells depicting borderline tuberculoid leprosy (BT), Whereas in TR mice, the macrophages showed foamy cytoplasmic changes depicting borderline lepromatous leprosy (BL). Other significant observations common to both non-TR and TR mice were: a) The lesions remained highly localized and showed signs of regression at the 6th and the 12th month intervals. b) The characteristic segmental demyelination and some attempt at remyelination were seen at the site. c) The influx of lymphocytes concorded well with demyelination. d) Bacteria were only seen in the macrophages and never in the Schwann cells or endothelial cells. e) Bacteria persisted in the macrophages, but appeared progressively degenerate at the 6th and 12th post-inoculation months, suggesting loss of viability. The study shows that there was a very effective containment of the infection and that the Schwann cells were resistant to M. leprae infection in the neural milieu. Nerve damage and Schwann cell bacillation do not go hand-in-hand. (AU)^ien. Descriptors: Nervo Ciático/anorm Nervo Ciático/les Nervo Ciático/fisiopatol Mycobacterium leprae/fisiol Mycobacterium leprae/ultraest Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2002/pdf/v70n1/v70n1a04.pdf / en Location: BR191.1

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Id: 19022 Author: Chaussinand, R. Title: Modifications mophologiques du bacille de Hansen, observées au cours des traitements antilépreux / Changes mophologiques bacillus Hansen, observed during anti-leprosy treatment Source: Rev. bras. Leprol;19(1):1-3, mar. 1951. . Descriptors: Mycobacterium leprae/fisiol Mycobacterium leprae/ultraest Hanseníase/fisiopatol Hanseníase/terap Limits: Humanos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/1951/PDF/v19n1a01.pdf / pt Location: BR191.1

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Id: 18419 Author: Patrocinio, Lucas Gomes; Goulart, Isabela Maria Bernardes; Goulart, Luiz Ricardo; Patrocinio, Jose Antonio; Ferreira, Frederico Regerio; Fleury, Raul Negrao Title: Detection of Mycobacterium leprae in nasal mucosa biopsies by the polymerase chain reaction ..- Source: s.l; s.n; 2005. 6 p. ilus, tab. Abstract: Several discoveries about leprosy indicate that Mycobacterium leprae transmission mainly accurs by inhalation, and the nose is major port of entry and exit. The present study evaluated the clinical application of PCR for detection of M. leprae DNA in nasal mucosa biopsies in untreated leprosy patients (52) and their contacts (99) from the State Reference Center in Sanitary Dermatology and Leprosy, Uberlandia, MG, Brazil. PCR detection of a 372-base pair DNA fragment from M. leprae was accomplished in 36 (69.2%) patients, from which 34 (91.9%) of them were multibacillaries. Furhermore, PCR was positive in 3 (16.7%) of 18 slit-skin smear negative, 4 (25.0%) of 16 skin lesion BI negative, 8 (33.3%) of 24 nasal mucosa BI negative patients, and 10 of 99 contacts (10.1%). The presence of bacilli in 10.1% of the contats may potentially reflect an occult leprosy, and reflect an occult leprosy, and these patients must be accompanied, followed by a chemoprophylaxy treatment. Considering all PCR results against clinical and BI classification of patients and controls, we have found a sensitivity of 69.2%, a specificity of 89.9% and an accuracy of 82.8%. It has been demonstrated here through PCR of nasal biopsies that the bacillus invades the mucosa, passing through the nasal inferior turbinate to reach peripheral blood. Therefore, the molecular investigation of invasive nasal biopsies by PCR tests has proven to be useful in defining patients of higher risk of transmission and risk-group contacts, which is an important step ti reach the World Health Organization objective towards the elimination of leprosy as a public health problem (AU). Descriptors: MUCOSA NASAL/imunol MUCOSA NASAL/les MUCOSA NASAL/microbiol MUCOSA NASAL/patol MYCOBACTERIUM LEPRAE/isol MYCOBACTERIUM LEPRAE/ultraest REACAO EM CADEIA DA POLIMERASE/estatíst REACAO EM CADEIA DA POLIMERASE/tend REACAO EM CADEIA DA POLIMERASE/util - BIOPSIA/estatíst  BIOPSIA/tend  BIOPSIA/util  HANSENIASE/clas  HANSENIASE/diag  HANSENIASE/microbiol  HANSENIASE/patol Limits: HUMANO Location: BR191.a; 09307/s

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Id: 18390 Author: Ebenezer, G. J; Norman, G; Joseph, G. A; Daniel, S; Job, C. K Title: Drug resistant-Mycobacterium leprae- results of mouse footpad studies from a laboratory in South India ..- Source: s.l; s.n; 2002. 12 p. tab. Abstract: Out of 265 biopsies of leprosy patients received at the Experimental Laboratory of Schieffelin Leprosy Research and Training Centre from 1987 to 1997 for evaluating resistant strains of M. leprae using the mouse footpad technique, 49 showed resistant strains of M. leprae to varying concentration of dapsone, rifampicin and clofazimine. 23 (47%) of these were from a control area. With 369 skin-smear positive multibacillary (MB) patients as the risk group (denominator), 23 (6.23%) were resistant to one or more drugs. 18 (4.88%) had dapsone resistance, 5 (1.36%) were resistant to rifampicin and 9 (2.44%) had resistance to low concentrations of clofazimine (0.0001%). Out of the 23 biopsies with drug resistance from the control area, primary dapsone resistance was seen in 7 (30%) biopsies and secondary dapsone resistance in 11 (48%). Primary rifampicin resitance was seen in 4 (17.4%) patients, secondary rifampicin resistance in 1 (4.35%) and primary clofazimine resistance in 7 (30%). 3 (13%) of the strains showed secondary clofazimine resistance. One biopsy had resistent strains to all the three drugs. In a control area where properly supervised effective multidrug therapy (MDT) was regularly administered over the years, the emergence of drug resistance is negligible. It may not be the case if the content, duration and regularity of the drug regimen were not satisfactory. Aware of the possible shortcomings in mass administration of MDT, it is emplasized that mouse footpad studies on drug resistance should be made available at least in endemic areas where the incidence of the disease has not changed despite good MDT coverage in order to monitor the emergence of drug resistance. Research into molecular biological identification of drug resistant-M.leprae should be intensified. These steps would help to institute timely measures to check the spread of any drug-resistant organisms in the community (AU). Descriptors: MYCOBACTERIUM LEPRAE/cresc MYCOBACTERIUM LEPRAE/isol MYCOBACTERIUM LEPRAE/metab MYCOBACTERIUM LEPRAE/patogen MYCOBACTERIUM LEPRAE/ultraest RESISTÊNCIA A DROGAS DAPSONA/uso terap CLOFAZIMINA/uso terap RIFAMPINA/uso terap TESTES DE SENSIBILIDADE MICROBIANA/métodos TESTES DE SENSIBILIDADE MICROBIANA/vet - HANSENIASE/clas  HANSENIASE/compl  HANSENIASE/quimioter  HANSENIASE/imunol  HANSENIASE/microbiol  HANSENIASE/patol  OFLOXACINO/uso terap  MINOCICLINA/uso terap Limits: HUMANO Location: BR191.1; 09299/s

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Id: 18349 Author: Delville, J Title: Acid-fast and non-acid-fast forms of the leprosy bacillus: implications in the diagnosis of leprosy ?- Source: s.l; s.n; 1979. 3p p. . Descriptors: MYCOBACTERIUM LEPRAE/citol MYCOBACTERIUM LEPRAE/isol MYCOBACTERIUM LEPRAE/metab MYCOBACTERIUM LEPRAE/patogen MYCOBACTERIUM LEPRAE/ultraest - HANSENIASE/etiol  HANSENIASE/imunol  HANSENIASE/microbiol Limits: IN VITRO Location: BR191.1; 00361/s

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Id: 15307 Author: Denney, O. E. Title: A microscopic study of mycobacterium leprae. Source: Int. J. Lepr;2(3):275-278, Aug.-Oct. 1934. ilus. Descriptors: MYCOBACTERIUM LEPRAE/citol MYCOBACTERIUM LEPRAE/isol MYCOBACTERIUM LEPRAE/ultraest Electronic Medium: http://www.ilsl.br Location: BR191.1

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Id: 15168 Author: Carpano, M Title: Su di una particolare colorazione e sulla morfologia del Bacillo della Lebbra ..- Source: São Paulo; s.n; set. 1940. 4 p. . Abstract: M. Carpano (Archivio Ital. di Anat. ed Istol Patol., v.III, N.I.,1936), il quale aveve già studiato la minuta morfologia dei bacilli della morva, della difterite e della tubercolosi sottoposti a particolari metodi di colorazione, espone in questa memoria-detta-gliattamente illustrata de 12 fotomicrorafie - i risultati ottenuti da ricerche consimili praticate su di un altro germe dello stesso grupo delle Mycobacteriaceae, e cioè sul M. leprae. (AU). Descriptors: MYCOBACTERIUM LEPRAE/citol MYCOBACTERIUM LEPRAE/ultraest Location: BR191.1; 0893/d.a

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Id: 13554 Author: Marchiondo Alan A; Smith, Jerome H; File, Sharon K Title: Naturally occurring leprosy-like disease of wild armadillos: ultrastructure of lepromatous lesions ..- Source: s.l; s.n; mar. 1980. 15 p. ilus. Abstract: An idependent survey of leprosy-like disease in wild armadillos was undertaken in the French Acadiana section of Louisiana in roder to arbitrate the controversy over the existence of this entity(21). As part of this study, material from lepromas was prepared for electron microscopy. Bacilli were concentrated in macrophages and were also found in capillary endothelial cells and fibroblasts, but tjey were not observed in lymphocytes or plasma cells. Bacilli consisted of electron-dense fibrillogranular material limited by a pentalaminar membrane (inner trilaminate plasma membrane and outer bilaminate cell wall). Bacillary division was common and was manifested as irregularly coiled nuclear strands and transverse septation by ingrowth of the plasma membrane. Degenerating bacilli were numerous in large multinucleate macrophages. The host inflammatory infiltrate was comprised of active plasma cells, untransformed lymphocytes, and macrophages originating from circulating monocytes. Monocytes recently emerging from capillaries were small and had a relative paucity of bacilli and lysosomes. These monocytes increased in size, plasma membrane complexity, bacillary burden, numbers and varieties of heterophagic and autophagic lysosomes, numbers of nuclei and nucleolar activity. Replicating, interphase and degenerating bacilli were found within macrophage phagolysosomes or free in the cytoplasmic matrix often aggreated in ranks forming "cigar bundles". Bacilli were variably surrounded by an eletronlucent substance comparable to the "gloae" or "schleim layer" described in human leprosy. Aggregates of bacilli in "gloae" formed "foamy bodies" and rarely observed opaqe droplets. thus, the ultrastructural features of this disease in wild armadillos are identical to those seen in human leprosy and armadillos experimentally infected with Mycobacterium leprae from human lesions. The findings suggest that B lymphocyte-macrophage interaction may be the predominant mechanism of leproma formation.(AU). Descriptors: TATUS/microbiol FIBROBLASTOS/microbiol HANSENIASE/microbiol HANSENIASE/patol HANSENIASE/vet MACROFAGOS/microbiol MACROFAGOS/ultraest MYCOBACTERIUM LEPRAE/ultraest XENARTROS/microbiol Limits: ANIMAL Electronic Medium: http://www.ilsl.br Location: BR191.1; 00937/s

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Id: 13434 Author: Saha, Kunal; Chakraborty, Asit K; Prakash, Nam Title: A quick method of demonstrating bacillaemia in patients with lepromatous leprosy and ultrastructural studies of the circulating acid-fast bacilli ..- Source: s.l; s.n; 1983. 5 p. ilus, tab. Abstract: While studying circulating immune complexes (CIC) in the sera of lepromatous patients by the polyethylene glycol (PEG) precipitation technique, we found (by light microscopy) abundant acid-fast bacilli (AFB), morphologically similar to those seen in slit skin smear preparations from these patients, precipitated with the PEG precipitated materials. Both solid and non-solid AFB could be readily identified. Ultrastructures of these AFB in the PEG aggregates showed some similarity with those detected in the PEG precipitates prepared from armadillo-derived lepromin under identical conditions. The most striking difference between the AFB in the test sera and that in the armadillo-derived lepromin was the absence of any transverse band in the former. This suggested that the AFB in the patients' circulation were not in division stage. Furthermore, electron-dense material were deposited on the AFB co-precipitated from the patients' sera, which were not found on the AFB co-precipitated from the armadillo-derived lepromin.(AU). Descriptors: TATUS TECNICAS BACTERIOLOGICAS SANGUE/microbiol ANTIGENO DE MITSUDA HANSENIASE/microbiol MICROSCOPIA ELETRÔNICA SEPTICEMIA/microbiol MYCOBACTERIUM LEPRAE/isol MYCOBACTERIUM LEPRAE/ultraest - PRECIPITACAO  POLIETILENOGLICOIS Limits: HUMANO ANIMAL ADULTO MEIA-IDADE IDOSO Electronic Medium: http://www.ilsl.br Location: BR191.1; 01767/s

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Id: 9643 Author: Khine, Michael Title: Foamy histiocytes and lepra bacilli in a lymph node aspirate ..- Source: s.l; s.n; 1993. 2 p. . Descriptors: BIOPSIA POR AGULHA HISTIOCITOS HANSENIASE GANGLIOS LINFATICOS MYCOBACTERIUM LEPRAE Location: BR191.1; 06596/s

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Id: 9267 Author: Esaguy, Nair; Aguas, Artur P; Van Embden, Jan D. A; Silva, Manuel T Title: Mycobacteria and human autoimmune disease: direct evidence of cross-reactivity between human lactoferrin and the 65-kilodalton protein of tubercle and leprosy bacilli ..- Source: s.l; s.n; 1991. 9 p. ilus. Descriptors: HANSENIASE LEPROMATOSA MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM TUBERCULOSIS MYCOBACTERIUM TUBERCULOSIS PELE PELE WESTERN BLOTTING PROTEINAS DO CHOQUE TÉRMICO IMUNOHISTOQUIMICA LACTOFERRINA Location: BR191.1; 06248/s

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Id: 8382 Author: Job, C. K; Chehl, S; Hastings, R. C; Ruby, J. R Title: Invasion of fiver parenchymal cells by Mycobacterium leprae in an experimentally infected nude mouse ..- Source: s.l; s.n; 1983. 8 p. ilus. Descriptors: GRANULOMA HANSENIASE HANSENIASE FIGADO FIGADO MICROSCOPIA ELETRONICA MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE FAGOCITOSE VACUOLOS Location: BR191.1; 02400/s

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Id: 8362 Author: Cree, I. A; Swanson Beck, J Title: The influence of killed Mycobacterium leprae and other mycobacteria on opsonized yeast phagocytosis ..- Source: s.l; s.n; 1986. 6 p. ilus, graf. Descriptors: MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE HANSENIASE AGREGAÇAO CELULAR LUMINESCENCIA MICROSCOPIA ELETRONICA MONOCITOS NEUTROFILOS OPSONINAS ZIMOSAN Location: BR191.1; 02421/s

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Id: 6952 Author: Hackel, Cristine. Title: Identificacao especifica do M. Leprae pela tecnica da reacao em cadeia da polimerase (PCR) / Specific detection of M. Leprae by technique polymerase chain reaction Source: Hansen. Int;15(1/2):67-75, jun.- dez. 1990. mapas, tab, graf. Abstract: A autora descreve a metodologia baseada na reaçäo em cadeia da polimerase (PCR) para identificaçäo específica do DNA de Mycobacterium leprae e apresenta uma revisäo das estratégias relacionadas à escolha dos oligonucleotídios iniciadores de síntese do DNA ("primers") e dos critérios de especificidade e sensibilidade necessários à utilizaçäo desse método em estudos clínicos e experimentais. (AU). Descriptors: HANSENIASE HANSENIASE HANSENIASE POLI A POLIMERASE - MYCOBACTERIUM LEPRAE  MYCOBACTERIUM LEPRAE  SISTEMAS DE NOTIFICAÇAO DE REAÇOES ADVERSAS A MEDICAMENTOS  REAÇAO EM CADEIA POR POLIMERASE Electronic Medium: http://hansen.bvs.ilsl.br/textoc/hansenint/v01aov20/1990/PDF/v15n1-2a06.pdf / pt Location: BR191.1

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Id: 6844 Author: Souza-Araujo, Heraclides Cesar de Title: Morphology of Mycobacterium leprae hominis and M. Leprae muris based on electron and phases contrast microscopy ..- Source: s.l; s.n; 1955. 2 p. . Descriptors: HANSENIASE - MYCOBACTERIUM LEPRAE  MICROSCOPIA ELETRONICA Location: BR191.1; 05165/s

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Id: 6734 Author: Edwards, Rosa P Title: An ultrastructural study of neuromuscular spindles in normal nice: with reference to mice and man infected with Mycobacterium leprae ..- Source: s.l; s.n; 1975. 19 p. tab, graf. Descriptors: HANSENIASE - MYCOBACTERIUM LEPRAE Location: BR191.1; 03872/s

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Id: 6668 Author: Souza-Araujo, Heraclides Cesar de Title: Sensibilidade da Macaca mulatta ao bacilo de Stefansky, amostra do Instituto Pasteur de Paris, e estudo anátomo-patológico das lesoes produzidas ..- Source: s.l; s.n; 1952. 10 p. ilus. Descriptors: HANSENIASE HANSENIASE - ALTERNATIVAS DE TESTES COM ANIMAIS  MYCOBACTERIUM LEPRAE  MYCOBACTERIUM LEPRAE Limits: ANIMAL Location: BR191.1; 05161/s

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Id: 6647 Author: Manalang, J Title: The morphology of Mycobacterium leprae: third report ..- Source: s.l; s.n; 1939. 9 p. tab. Descriptors: MYCOBACTERIUM LEPRAE BACTERIOLOGIA Location: BR191.1; 05104/s

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